Journal: Aging (Albany NY)

Article Title: D-galactose causes sinoatrial node dysfunction: from phenotype to mechanism

doi: 10.18632/aging.205196

Figure Lengend Snippet: Structural formula of D-galactose.

Article Snippet: D-galactose (T0591, purity: 99.84%) was purchased from Topscience Medicine Technology (China), while C57BL/6 mice were purchased from Changsheng Biotechnology (China, Production License: SCXK (Liao) 2020-0001), and raised with no dietary restrictions.

Techniques:

Journal: Aging (Albany NY)

Article Title: D-galactose causes sinoatrial node dysfunction: from phenotype to mechanism

doi: 10.18632/aging.205196

Figure Lengend Snippet: D-galactose induces senescence in mice. ( A ) D-galactose causes hair senescence in mice. ( B ) D-galactose causes weight gain in mice. NS represents P >0.05; ** represents P <0.01; Control: control group; D-galactose: D-galactose administered group.

Article Snippet: D-galactose (T0591, purity: 99.84%) was purchased from Topscience Medicine Technology (China), while C57BL/6 mice were purchased from Changsheng Biotechnology (China, Production License: SCXK (Liao) 2020-0001), and raised with no dietary restrictions.

Techniques: Control

Journal: Aging (Albany NY)

Article Title: D-galactose causes sinoatrial node dysfunction: from phenotype to mechanism

doi: 10.18632/aging.205196

Figure Lengend Snippet: D-galactose decreased SAN and heart function in mice. ( A ) Electrocardiogram. ( B ) Echocardiogram. ( C ) Cerebral blood flux. ( D ) Heart rate in the indicated groups. ( E , F ) LVEF and LVFS in the indicated groups. ( G ) BNP assay results for the indicated groups. ( H ) Cerebral blood flux results for the indicated groups. * represents P <0.05; ** represents P <0.01; Control: control group; D-galactose: D-galactose administered group.

Article Snippet: D-galactose (T0591, purity: 99.84%) was purchased from Topscience Medicine Technology (China), while C57BL/6 mice were purchased from Changsheng Biotechnology (China, Production License: SCXK (Liao) 2020-0001), and raised with no dietary restrictions.

Techniques: B-type Natriuretic Peptide Assay, Control

Journal: Aging (Albany NY)

Article Title: D-galactose causes sinoatrial node dysfunction: from phenotype to mechanism

doi: 10.18632/aging.205196

Figure Lengend Snippet: D-galactose causes fibrosis of sinoatrial node and myocardium. ( A ) Masson’s trichrome staining of sinoatrial node (Scale = 50 μm) and myocardium (Scale = 20 μm). ( B ) CVF of sinoatrial node. ( C ) CVF of myocardium. Black arrow represents the artery of sinoatrial node; ** represents P <0.01; Control: control group; D-galactose: D-galactose administered group.

Article Snippet: D-galactose (T0591, purity: 99.84%) was purchased from Topscience Medicine Technology (China), while C57BL/6 mice were purchased from Changsheng Biotechnology (China, Production License: SCXK (Liao) 2020-0001), and raised with no dietary restrictions.

Techniques: Staining, Control

Journal: Aging (Albany NY)

Article Title: D-galactose causes sinoatrial node dysfunction: from phenotype to mechanism

doi: 10.18632/aging.205196

Figure Lengend Snippet: D-galactose induces oxidative stress in the sinoatrial node. ( A , B ) DHE staining results for the indicated groups. Scale = 50 μm. ( C , D ) SOD and MDA assay results for the indicated groups. * represents P <0.05; ** represents P <0.01; Control: control group; D-galactose: D-galactose administered group.

Article Snippet: D-galactose (T0591, purity: 99.84%) was purchased from Topscience Medicine Technology (China), while C57BL/6 mice were purchased from Changsheng Biotechnology (China, Production License: SCXK (Liao) 2020-0001), and raised with no dietary restrictions.

Techniques: Staining, Multiple Displacement Amplification, Control

Journal: Aging (Albany NY)

Article Title: D-galactose causes sinoatrial node dysfunction: from phenotype to mechanism

doi: 10.18632/aging.205196

Figure Lengend Snippet: Oxidative stress causes I f channel abnormality. ( A ) Immunofluorescence staining of the SAN. Scale = 100 μm. ( B ) Western blotting assay. ( C ) Results of immunofluorescence staining. ( D , E ) Results of Western blotting assay. * represents P <0.05; ** represents P <0.01; Control: control group; D-galactose: D-galactose administered group.

Article Snippet: D-galactose (T0591, purity: 99.84%) was purchased from Topscience Medicine Technology (China), while C57BL/6 mice were purchased from Changsheng Biotechnology (China, Production License: SCXK (Liao) 2020-0001), and raised with no dietary restrictions.

Techniques: Immunofluorescence, Staining, Western Blot, Control

Journal: Aging (Albany NY)

Article Title: D-galactose causes sinoatrial node dysfunction: from phenotype to mechanism

doi: 10.18632/aging.205196

Figure Lengend Snippet: D-galactose causes cell senescence. ( A , B ) Results of P16 immunofluorescence staining. Scale = 20 μm. ( C , D ) Results of β-galactosidase staining. Scale = 100 μm. ** represents P <0.01; Control: control group; D-galactose: D-galactose administered group; Edaravone: D-galactose+Edaravone administered group.

Article Snippet: D-galactose (T0591, purity: 99.84%) was purchased from Topscience Medicine Technology (China), while C57BL/6 mice were purchased from Changsheng Biotechnology (China, Production License: SCXK (Liao) 2020-0001), and raised with no dietary restrictions.

Techniques: Immunofluorescence, Staining, Control

Journal: Aging (Albany NY)

Article Title: D-galactose causes sinoatrial node dysfunction: from phenotype to mechanism

doi: 10.18632/aging.205196

Figure Lengend Snippet: D-galactose causes cell peroxidation and decreased mitochondrial function. ( A ) Mito-tracker and DCFH-DA staining results for the indicated groups. Scale = 20 μm. ( B , C ) Staining results for the indicated groups. * represents P <0.05; ** represents P <0.01; Control: control group; D-galactose: D-galactose administered group; Edaravone: D-galactose+Edaravone administered group.

Article Snippet: D-galactose (T0591, purity: 99.84%) was purchased from Topscience Medicine Technology (China), while C57BL/6 mice were purchased from Changsheng Biotechnology (China, Production License: SCXK (Liao) 2020-0001), and raised with no dietary restrictions.

Techniques: Staining, Control

Journal: Aging (Albany NY)

Article Title: D-galactose causes sinoatrial node dysfunction: from phenotype to mechanism

doi: 10.18632/aging.205196

Figure Lengend Snippet: D-galactose causes cell cycle and proliferation abnormalities. ( A , B ) Results of cell cycle assay. ( C ) CCK-8 assay results for the indicated groups. NS represents P >0.05; * represents P <0.05; ** represents P <0.01; Control: control group; D-galactose: D-galactose administered group; Edaravone: D-galactose+Edaravone administered group.

Article Snippet: D-galactose (T0591, purity: 99.84%) was purchased from Topscience Medicine Technology (China), while C57BL/6 mice were purchased from Changsheng Biotechnology (China, Production License: SCXK (Liao) 2020-0001), and raised with no dietary restrictions.

Techniques: Cell Cycle Assay, CCK-8 Assay, Control

Journal: Aging (Albany NY)

Article Title: D-galactose causes sinoatrial node dysfunction: from phenotype to mechanism

doi: 10.18632/aging.205196

Figure Lengend Snippet: D-galactose causes abnormal expression of pacing-related gene. ( A – D ) RT-qPCR results for the indicated groups. * represents P <0.05; ** represents P <0.01; Control: control group; D-galactose: D-galactose administered group; Edaravone: D-galactose+Edaravone administered group.

Article Snippet: D-galactose (T0591, purity: 99.84%) was purchased from Topscience Medicine Technology (China), while C57BL/6 mice were purchased from Changsheng Biotechnology (China, Production License: SCXK (Liao) 2020-0001), and raised with no dietary restrictions.

Techniques: Expressing, Quantitative RT-PCR, Control

Journal: Aging (Albany NY)

Article Title: D-galactose causes sinoatrial node dysfunction: from phenotype to mechanism

doi: 10.18632/aging.205196

Figure Lengend Snippet: ROS scavenging and Pitx2 silencing improves pacemaker ion channel. ( A , B ) Immunofluorescence staining results for the indicated groups. Scale = 20 μm. * represents P <0.05; ** represents P <0.01; Control: control group; D-galactose: D-galactose administered group; Edaravone: D-galactose+Edaravone administered group; si-NC: si-NC transfection+D-galactose administered group; si- Pitx2 : si- Pitx2 transfection+D-galactose administered group.

Article Snippet: D-galactose (T0591, purity: 99.84%) was purchased from Topscience Medicine Technology (China), while C57BL/6 mice were purchased from Changsheng Biotechnology (China, Production License: SCXK (Liao) 2020-0001), and raised with no dietary restrictions.

Techniques: Immunofluorescence, Staining, Control, Transfection

Journal: Aging (Albany NY)

Article Title: D-galactose causes sinoatrial node dysfunction: from phenotype to mechanism

doi: 10.18632/aging.205196

Figure Lengend Snippet: Results of Western blotting assay. ( A , B ) Results of Western blotting and statistical analysis. * represents P<0.05; ** represents P<0.01; Control: control group; D-galactose: D-galactose-administered group; Edaravone: D-galactose+Edaravone-administered group; si-NC: si-NC transfection + D-galactose-administered group; si-Pitx2: si-Pitx2 transfection + D-galactose-administered group.

Article Snippet: D-galactose (T0591, purity: 99.84%) was purchased from Topscience Medicine Technology (China), while C57BL/6 mice were purchased from Changsheng Biotechnology (China, Production License: SCXK (Liao) 2020-0001), and raised with no dietary restrictions.

Techniques: Western Blot, Control, Transfection

Journal: Aging (Albany NY)

Article Title: D-galactose causes sinoatrial node dysfunction: from phenotype to mechanism

doi: 10.18632/aging.205196

Figure Lengend Snippet: ROS scavenging and PITX2 silencing improves pulsatile function in hiPSC-CMs. ( A ) Field potential traces of hiPSC-AMs at 48h. ( B , C ) Relative trend of beat rate and field potential duration. ( D , E ) Beat rate and field potential duration of hiPSC-AMs at 48h. * represents P <0.05; ** represents P <0.01; Control: control group; D-galactose: D-galactose-administered group; Edaravone: D-galactose+Edaravone-administered group; si-NC: si-NC transfection+D-galactose administered group; si- PITX2 : si- PITX2 transfection+D-galactose-administered group.

Article Snippet: D-galactose (T0591, purity: 99.84%) was purchased from Topscience Medicine Technology (China), while C57BL/6 mice were purchased from Changsheng Biotechnology (China, Production License: SCXK (Liao) 2020-0001), and raised with no dietary restrictions.

Techniques: Control, Transfection

Journal: Annals of Translational Medicine

Article Title: The effect of low frequency and low intensity ultrasound combined with microbubbles on the sonoporation efficiency of MDA-MB-231 cells

doi: 10.21037/atm.2020.02.155

Figure Lengend Snippet: Factors and levels of the experimental protocol

Article Snippet: Microbubble dose, irradiation time, and acoustic intensity were adjusted to observe differences between the bioeffects demonstrated. table ft1 table-wrap mode="anchored" t5 caption a7 Level Factors Sound intensity (a), mW/cm 2 Irradiation time (b), min Microbubble dose (c), mL 1 230 1 0 2 300 2 0.2 3 370 3 0.4 4 0.6 5 0.8 6 1 Open in a separate window caption a8 Factors and levels of the experimental protocol Fluorescence positive rate and cell survival rate After irradiation, the cells are washed with PBS 3 times, and the entry of fluorescent reagent FD4 at different treatment levels was observed under the EVOSTM M5000 Imaging System (Thermo Fisher Science, USA).

Techniques: Irradiation

Journal: Annals of Translational Medicine

Article Title: The effect of low frequency and low intensity ultrasound combined with microbubbles on the sonoporation efficiency of MDA-MB-231 cells

doi: 10.21037/atm.2020.02.155

Figure Lengend Snippet: FD4 fluorescent positive cells showed bright green fluorescence in the fluorescence mode, while non-stained cells did not. Fluorescence staining results in the blank control group, ultrasound irradiation groups and ultrasound irradiation combined with microbubble group are shown in A,B,C,D,E,F,G: (A) fluorescence staining result in the blank control group that microbubbles and ultrasound irradiation are not added; (B,C,D,E,F,G): irradiated by ultrasound (370 mW/cm2, 1 minute) and 0, 0.2, 0.4, 0.6, 0.8, and 1 mL microbubbles are added respectively.

Article Snippet: Microbubble dose, irradiation time, and acoustic intensity were adjusted to observe differences between the bioeffects demonstrated. table ft1 table-wrap mode="anchored" t5 caption a7 Level Factors Sound intensity (a), mW/cm 2 Irradiation time (b), min Microbubble dose (c), mL 1 230 1 0 2 300 2 0.2 3 370 3 0.4 4 0.6 5 0.8 6 1 Open in a separate window caption a8 Factors and levels of the experimental protocol Fluorescence positive rate and cell survival rate After irradiation, the cells are washed with PBS 3 times, and the entry of fluorescent reagent FD4 at different treatment levels was observed under the EVOSTM M5000 Imaging System (Thermo Fisher Science, USA).

Techniques: Fluorescence, Staining, Control, Irradiation

Journal: Annals of Translational Medicine

Article Title: The effect of low frequency and low intensity ultrasound combined with microbubbles on the sonoporation efficiency of MDA-MB-231 cells

doi: 10.21037/atm.2020.02.155

Figure Lengend Snippet: Positive rate of cell fluorescence staining at different levels of 3 factors: x-positive rate of fluorescence staining, (A) ultrasound intensity, (B) irradiation time, (C) microbubble dose.

Article Snippet: Microbubble dose, irradiation time, and acoustic intensity were adjusted to observe differences between the bioeffects demonstrated. table ft1 table-wrap mode="anchored" t5 caption a7 Level Factors Sound intensity (a), mW/cm 2 Irradiation time (b), min Microbubble dose (c), mL 1 230 1 0 2 300 2 0.2 3 370 3 0.4 4 0.6 5 0.8 6 1 Open in a separate window caption a8 Factors and levels of the experimental protocol Fluorescence positive rate and cell survival rate After irradiation, the cells are washed with PBS 3 times, and the entry of fluorescent reagent FD4 at different treatment levels was observed under the EVOSTM M5000 Imaging System (Thermo Fisher Science, USA).

Techniques: Fluorescence, Staining, Irradiation

Journal: Annals of Translational Medicine

Article Title: The effect of low frequency and low intensity ultrasound combined with microbubbles on the sonoporation efficiency of MDA-MB-231 cells

doi: 10.21037/atm.2020.02.155

Figure Lengend Snippet: Statistical analysis results (P values) of the 3 factors on the fluorescence staining positive rate respectively. a, ultrasound intensity; b, irradiation time; c, microbubble dose.

Article Snippet: Microbubble dose, irradiation time, and acoustic intensity were adjusted to observe differences between the bioeffects demonstrated. table ft1 table-wrap mode="anchored" t5 caption a7 Level Factors Sound intensity (a), mW/cm 2 Irradiation time (b), min Microbubble dose (c), mL 1 230 1 0 2 300 2 0.2 3 370 3 0.4 4 0.6 5 0.8 6 1 Open in a separate window caption a8 Factors and levels of the experimental protocol Fluorescence positive rate and cell survival rate After irradiation, the cells are washed with PBS 3 times, and the entry of fluorescent reagent FD4 at different treatment levels was observed under the EVOSTM M5000 Imaging System (Thermo Fisher Science, USA).

Techniques: Fluorescence, Staining, Irradiation

Journal: Annals of Translational Medicine

Article Title: The effect of low frequency and low intensity ultrasound combined with microbubbles on the sonoporation efficiency of MDA-MB-231 cells

doi: 10.21037/atm.2020.02.155

Figure Lengend Snippet: The optimal parameter combination of the 3 factors. (A) The positive rate of fluorescence at different sound intensities when the irradiation time was 2 minutes and 0.6 mL of microbubbles were added; (B) the positive rate of fluorescence at different irradiation times when the ultrasound intensity and microbubbles were 300 mW/cm2 and 0.6 mL respectively; (C) the positive rate of fluorescence at different microbubble concentrations when ultrasound intensity and irradiation time were 300 mW/cm2 and 2 minutes respectively.

Article Snippet: Microbubble dose, irradiation time, and acoustic intensity were adjusted to observe differences between the bioeffects demonstrated. table ft1 table-wrap mode="anchored" t5 caption a7 Level Factors Sound intensity (a), mW/cm 2 Irradiation time (b), min Microbubble dose (c), mL 1 230 1 0 2 300 2 0.2 3 370 3 0.4 4 0.6 5 0.8 6 1 Open in a separate window caption a8 Factors and levels of the experimental protocol Fluorescence positive rate and cell survival rate After irradiation, the cells are washed with PBS 3 times, and the entry of fluorescent reagent FD4 at different treatment levels was observed under the EVOSTM M5000 Imaging System (Thermo Fisher Science, USA).

Techniques: Fluorescence, Irradiation

Journal: Molecules

Article Title: Synthesis of Some Mono- and Disaccharide-Grafting Phthalazine Derivatives and Some New Se -Nucleoside Analogues: Antibacterial Properties, Quantum Chemical Calculations, and Cytotoxicity

doi: 10.3390/molecules28010317

Figure Lengend Snippet: Synthesis of 4-(pyren-1-ylmethyl)-1-( d -glycosyloxy) phthalazine nucleosides 11a , b and 13 . Reagents and conditions: ( i ) HMDS, (NH 4 ) 2 SO 4 ; ( ii ) 1,2,3,4,6-penta- O -acetyl- α - d -glucopyranose or α- d -galactopyranose pentaacetate, SnCl 4 , ethylene chloride; ( iii ) β - d -ribopyranosetetraacetate, (i v ) SnCl 4 , ( v ) Et 3 N, MeOH.

Article Snippet: Acetobromo-α- d -xylose, stabilized with 2.5% CaCO 3 , α- d -Galactose pentaacetate 98%, d -glucono-1,5-lactone and d -glucoheptonic acid-1,4-lactone from Biosynth Carbosynth ® (Manchester, UK). acetobromo-α- d -lactose 95% by HPLC, from Creative Biolabs (Shirley, NY, USA).

Techniques: